Measurement

Part:BBa_K173015:Design

Designed by: Lorenzo Pasotti, Manuel Lupotto, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

HSL inducible lysis device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2419
    Illegal NheI site found at 2442
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2024
    Illegal AgeI site found at 2094
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal SapI site found at 2675


Design Notes

We used BioBrick Standard Assembly.


Source

BBa_F2620, iGEM Headquarters;

BBa_K112808, 2009 DNA Distribution.

References

[1] Barry Canton, Anna Labno, and Drew Endy. Refinement and standardization of synthetic biological parts and devices. Nature biotechnology, 26(7):787-793, 2008.

[2] Pubmed Accession Number: NC_000866 Enterobacteria phage T4.